Another Important Contribution to the Scientific World from QuoData: "In-house validation of a droplet digital PCR system using a multifactorial experimental design"

QuoData is proud to announce another significant scientific contribution with the publication of “In-house validation of a droplet digital PCR system using a multifactorial experimental design”. In this study, Steffen Uhlig and Bertrand Colson, together with other scientists, present a robust, real-world approach to validating droplet digital PCR (ddPCR) systems.

Unlike traditional validation, which often focuses narrowly on performance KPIs, this work demonstrates that method validation can — and should — ensure a method truly works under the varied conditions of everyday laboratory use. The team’s multifactorial design rigorously tested the Bio-Rad QX200 ddPCR system across multiple parameters, confirming its robustness and reliability. Notably, the study identified the choice of master mix and droplet handling as critical for accurate DNA quantification, while most other factors (such as operator or pipette type) had minimal impact.

As Mr. Uhlig emphasizes, “Method validation can do more — it can ensure that a method ‘works’ and not only map performance KPIs.” This forward-thinking approach is now a blueprint for labs aiming for both compliance and scientific excellence, and it is transferable to other digital PCR technologies. This publication underscores QuoData’s ongoing commitment to scientific innovation and practical solutions for the global laboratory community.

Read the full article: https://www.sciencedirect.com/science/article/pii/S0003267025005665?via%3Dihub